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KMID : 0364219960390010075
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Korean Journal of Zoology
1996 Volume.39 No. 1 p.75 ~ p.88
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Control of X Chromosome Reactivation and Determination of the Ratio of Sex Chromosome to Autosome in Embryonal Carcinoma Cell-Somatic Cell Hybrids
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Lee Kwang-Ho
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Abstract
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OTF9-63 (OTF9) and P19S101A1 (P19) embryonal carcinoma (EC) cells were examined for their ability to produce the reactivation of inactive X chromosomes from somatic cells. They were hybridized with various somatic cells and resulting HAT^(r) EC-somatic cell colnes were analysed for their morphology, chromosomal replication patterns and exprsion profiles of X-linked and distantly located genes, Hprt and Pgk-I. The results demonstrated that ORF9 cells could reactivate the inactive X-chromosome whereas P19 cells could not. In adition, EC-somatic cell hybrids tended to reduce the number of sex chromosomes in long-term culture, resulting in 1:2 ratio of sex chromosomes to autosomes. The use of EC cell hybrids provides an experimental system for studying the mechanism(s) of the X-reactivatio that is initiated and maintained from meiotic prophase of oogenesis to early embry genesis.
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KEYWORD
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Embryonal Carcinoma (EC) Cell Lines, Somatic Cell Hybridization, X Chromosome Reactivation, X Chromosome Loss, Y Chromosome Stabiligy, Ratio of Sex Chromosome:Autosome
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